Certified Agilent Services
We value Agilent microarrays
Our facility is committed to excellence and offers the most
suitable microarray solution for each study. We have extended
our microarray expertise to become an Agilent Certified Service
Provider because the Agilent platform is a robust, well validated
technology and the design of the microarrays is highly customizable.
Customers can benefit from high-quality 60mer oligo probe sets
for multiple genomes, multiplex slide formats and applications.
Our extensive experience in microarray applications combined
with Agilent’s advanced technology guarantee the quality
of the data for publication. The process is simple: send us
your RNA or DNA samples, and we will send you high quality data
for standard or custom Agilent arrays.
Our Certified Agilent Applications
Our facility is certified for one and two-colour gene expression
and microarray-based comparative genomic hybridization (aCGH)
and we are looking forward to be certified for miRNA and ChIP
shortly.
Our Agilent services
We provide RNA quality control, microarray processing for certified
applications, and data analysis. We also provide extended services
including experimental design consultation, custom microarray
design with eArray, RNA or DNA extraction, personalized data
analysis and data validation. We welcome projects of all sizes.
Pilot projects
If you’re new to Agilent or microarray technology, we’ll
use your samples to convince you of the value and quality of
the Agilent platform.
Software
We use the Agilent microarray scanner with its Feature Extraction
software, CGH Analytics, GeneSpring, R and Bioconductor and
Ingenuity Pathway Analysis.
About Data Analysis
We offer side-by-side guidance and training in all areas whether
it’s instruction on how to use the latest versions of cutting
edge software suites or customized statistical analysis for
presentations and publication. We also offer assistance in publishing
your data to public microarray data repositories such as NCBI’s
Gene Expression Omnibus (GEO) in MIAME compliant form. Our comprehensive
microarray analysis support and bioinformatics services include:
• data normalization
• custom-tailored analyses for individual questions
• statistical expertise in discussion of results
• various statistical analyses for differentially expressed
genes
• various visualizations of data,
• clustering analyses (all commonly used methods)
• the incorporation of annotations, gene ontology (GO)
information, and functional group analysis
• finding and visualizing pathways for differentially expressed
genes
Workflows
Typical Experimental Workflow for Gene Expression:
• Sample QC - with the Agilent 2100 Bioanalyzer
• Sample Labeling – one or two-colour labeling with
Agilent Microarray reagents and protocols
• Hybridization - in SureHyb chambers and the Agilent hybridization
oven
• Scanning and analysis - on the Agilent DNA microarray
scanner with Feature Extraction Software
• Final data analysis - using GeneSpring software
Typical Experimental Workflow for aCGH:
• Sample Labeling – two-colour labeling with Agilent
Microarray reagents and protocols
• Hybridization - in SureHyb chambers and the Agilent hybridization
oven
• Scanning and analysis - on the Agilent DNA microarray
scanner with Feature Extraction Software
• Final data analysis - using CGH Analytics software
Attended Conferences
• World Microarray Congress in Vancouver, Canada June 28-29
2008 (Poster presentation)
• Advance in Microarray Technology in Barcelona, Spain,
7-8 May 2008 (Poster presentation)
• Plant and Animal Genome Conference in San Diego, USA,
12-16 January 2008 (Booth #100).
• Prostate Cancer Conference 2007 in Toronto, Canada, 29-30
September 2007 (Poster presentation)
• World Microarray Congress in Vancouver, Canada June 29-30
2007 (Poster presentation)
>click here for information
on how to acknowledge the Microarray Facility for publications
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